0911111111

Protease and Lipase production of psychrotrophic bacteria of dairy origin

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Maymouna E. Osman* and M.C. Allan

Department ofApplied Microbiology, Strathclyde University, Glasgow.
United Kingdom

SUMMARY

Psychrotrophic bacteria were isolated from raw milk samples by incubation at 5°C — 7°C. Viable counts of these bacteria were carried out and visible colony forming units were found after 7days and 10 days at 5°C — 7°C. Isolates were tested for production of the extra-cellular enzyme lipase and protease. In each case enzyme positive cultures were purified and tested to identify to a generic level. The optimum temperature for enzyme production was tested for and was generally 30°C. The temperature at which the organisms were grown had effect on enzyme production.

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