Protease and Lipase production of psychrotrophic bacteria of dairy origin

Abstract

Psychrotrophic bacteria were isolated from raw milk samples by 
incubation at 5°C — 7°C. Viable counts of these bacteria were carried out and 
visible colony forming units were found after 7days and 10 days at 5°C — 
7°C. Isolates were tested for production of the extra-cellular enzyme lipase and 
protease. In each case enzyme positive cultures were purified and tested to 
identify to a generic level. The optimum temperature for enzyme 
production was tested for and was generally 30°C. The temperature at which 
the organisms were grown had effect on enzyme production.